TMEM184B Knockout cell line (COS-1)

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location： Home > Products > KO Cell Lines > TMEM184B Knockout cell line (COS-1)

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TMEM184B Knockout cell line (COS-1) Order

TMEM184B Knockout cell line (COS-1)
Catalog#
YKO-R065
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
TMEM184B Knockout cell line (COS-1)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
TMEM184B Knockout cell line (COS-1)
Morphology
Epithelial-like, adherent
Passage ratio
1:3-1:4
Species
Green monkey
Gene Name
TMEM184B
Gene ID
103223310
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10%FBS
Target region
Knockout region: Exon 3 (based on transcript TMEM184B-201)
Validation: PCR+Sanger Sequencing
TMEM184B Knockout cell line (COS-1) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the TMEM184B gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the COS-1 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, TMEM184B gene knockout TMEM184B Knockout cell line (COS-1) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio

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